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Parietichytrium sp. SEK364 was identified as Parietichytrium sarkarianum by analyses of 18S rRNA gene, morphology and cultural properties47. The Parietichytrium strains used in Every single experiment are indicated during the legend of each Figure and Table.
The reverse genetic strategy along with a tracing experiment using steady isotope-labeled fatty acids revealed the ELO/DES pathway is the only DHA synthesis pathway in Parietichytrium sp. Disruption of your C20 fatty acid ELO (C20ELO) and ∆4 fatty acid DES (∆4DES) genes with expression of ωthree fatty acid DES In this particular thraustochytrid authorized the manufacture of EPA and n-3docosapentaenoic acid (n-3DPA), respectively, at the highest stage amongst recognised microbial sources using fed-batch lifestyle.
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